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324713

Glu-C Protease

Selectively cleaves at glutamic acid residues, suitable for mass spectromety, from Staphylococcus aureus

Synonym(s):

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus, V8 Protease

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About This Item

CAS Number:
66676-43-5
EC Number:
3.4.21.19 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD01324998
eCl@ss:
32160410
Specific activity:
≥10 units/mg protein
Biological source:
Staphylococcus aureus
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Product Name

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus,

biological source

Staphylococcus aureus

Quality Segment

grade

Proteomics Grade

form

lyophilized

specific activity

≥10 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

protein mass spectrometry: suitable

shipped in

ambient

storage temp.

2-8°C

General description

Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA69, 3506.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

324713-UG: + 324713-50UG:

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