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324715

Lys-C Protease

Precise cleavage at lysine residues, suitable for mass spectrometry, from Lysobacter enzymogenes

Synonym(s):

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

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About This Item

CAS Number:
72561-05-8
EC Number:
3.4.21.50 (BRENDA, IUBMB)
UNSPSC Code:
12352202
MDL number:
MFCD02253020
NACRES:
NA.54
Specific activity:
≥200 units/mg protein
Assay:
≥90% (SDS-PAGE)
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Product Name

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes,

grade

Proteomics Grade

Quality Segment

assay

≥90% (SDS-PAGE)

form

lyophilized

specific activity

≥200 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic)

technique(s)

protein mass spectrometry: suitable

shipped in

ambient

storage temp.

2-8°C

General description

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Physical form

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Reconstitute in 50 µl of distilled H₂O.

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

324715-5UG: + 324715-5RUG:

jan

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