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343222

Biotin, Fluorescein Conjugate

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About This Item

NACRES:
NA.41
UNSPSC Code:
41116133
Technical Service
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biological source

(Synthetic chemical)

Quality Level

100

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

dilution

(EIA (1:1,000)
Tissue Staining (1:50 - 1:100))

color

orange

solubility

DMF: 5 mg/mL, DMSO: 5 mg/mL, methanol: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

It can be used for the detection of avidin and streptavidin.

Application

EIA (1:1,000)

Tissue Staining (1:50 - 1:100)

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Other Notes

Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203
Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

343222-1MG: + 343222-MG:

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Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
343222-1MGCN04055977195088