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8.51086

Oxyma Pure

≥99.0% (HPLC), for peptide synthesis, Novabiochem®

Synonym(s):

Oxyma Pure, Ethyl cyano(hydroxyimino)acetate

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About This Item

Empirical Formula (Hill Notation):
C5H6N2O3
CAS Number:
3849-21-6
Molecular Weight:
142.11
UNSPSC Code:
12352126
EC Index Number:
223-351-3
NACRES:
NA.22
MDL number:
MFCD00002112
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Product Name

Oxyma Pure, Novabiochem®

Quality Segment

300

product line

Novabiochem®

assay

≥99.0% (HPLC)

form

crystalline powder

reaction suitability

reaction type: Coupling Reactions

manufacturer/tradename

Novabiochem®

mp

130-132 °C

application(s)

peptide synthesis

storage temp.

2-8°C

InChI

1S/C5H6N2O3/c1-2-10-5(8)4(3-6)7-9/h9H,2H2,1H3/b7-4+

InChI key

LCFXLZAXGXOXAP-QPJJXVBHSA-N

General description

Oxyma Pure (Ethyl cyano(hydroxyimino)acetate) is a non-explosive alternative to the canonical peptide coupling additive N-hydroxybenzotriazole (HOBt). When used in place of HOBt in carbodiimide-mediated peptide bond formation, it provides products in higher yields with less racemization. The combination of Oxyma Pure/diisopropylcarbodiimide (DIC) has proven to be particularly effective in microwave-assisted peptide synthesis.
The generation of toxic hydrogen cyanide (HCN) from the reaction between Oxyma Pure and DIC has been observed. Fortunately, this side reaction can be eliminated by substituting DIC for the more hindered t-butylethylcarbodiimide.

Associated Protocols and Technical Articles
Guide to Selection of Coupling Reagents

Literature references:
[1] R. Subirós-Funosas, et al. (2009) Chem. Eur. J., 15, 9394
[2] J. Collins , et al. (2014) Org . Lett ., 16, 940.
[3] A. D. McFarland (2019) Org. Process Res. Dev., 23, 2099.
[4] S. R. Manne, et al (2022) Org. Process Res. Dev., 26, 2894.

Application

Further applications of Oxyma Pure:
  • Use in the synthesis of difficult peptides.
  • As an acidic modifier to prevent aspartimide formation.

Features and Benefits

  • Can be used in place of HOBt in carbodiimide-mediated coupling reactions without change of protocol
  • It gives results comparable to HOAt in step-wise solid-phase synthesis
  • Less epimerization than HOBt in fragment condensation reactions

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): crystalline powder
Assay (HPLC, area%): ≥ 99.0 % (a/a)
Identity (IR): passes test
Solubility (12,5 mmol in 25 ml DMF): clearly soluble

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

8510860250:4.548173382661E12 + 8510860025:4.548173382647E12 + 8510860100:4.548173382654E12 + 8510861000:4.548173383002E12 + 8510860500:4.548173383552E12

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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