Anti-Collagen Type I (COL1A1) Antibody

Collagen alpha-1(I) chain (UniProt: P02454; also known as Alpha-1 type I collagen) is encoded by the Col1a1 gene (Gene ID: 29393) in rat. Collagen alpha-1(I) chain (Col1a1) is a fibril-forming structural protein predominantly found in connective tissues, including bone, cornea, dermis, and tendon. Type I collagen is composed of two alpha-1 chains and one alpha-2 chain, forming a triple helical structure that provides tensile strength and mechanical stability to tissues. It functions as an extracellular matrix constituent, conferring rigidity and facilitating cellular responses to mechanical stimuli and signaling pathways such as collagen-activated tyrosine kinase receptor signaling. It is localized in the extracellular space and secretory granules, playing a key role in extracellular matrix assembly and maintenance. The expression of Col1a1 is regulated under various physiological and pathological conditions. It is upregulated during tissue remodeling processes, such as wound healing and fibrosis, and plays a role in pathological states, including myocardial infarction where it contributes to infarct healing through extracellular matrix remodeling. Aberrant expression or mutations in the Col1a1 gene are implicated in diseases such as osteogenesis imperfecta, Ehlers-Danlos syndrome, and idiopathic osteoporosis. Increased Col1a1 expression has been linked to processes like epithelial-to-mesenchymal transition (EMT), cancer progression, and invasion in conditions such as cholangiocarcinoma, making it a potential biomarker for disease severity and prognosis. Type I collagen is widely used in cartilage tissue engineering. (Ref.: Hou, L. et al., (2021) J Cancer. 12(19), 5723-5731; Jiang YH, et al., (2022) Am J Transl Res., 14(2), 1146-1159).

Target molecular weight ~ 150 kDa observed. 137.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Rat tail collagen

Anti-Collagen Type I (COL1A1) is a rabbit polyclonal antibody that detects rat and mouse Collagen alpha-1(I) chain.

Enzyme Immunoassay: A representative lot of this antibody detected rat and mouse collagen Type I, but not human collagen in ELISA application.

Dot Blot Analysis: A 1:500 dilution from a representative lot detected rat and mouse tail collagen, but not human placenta collagen.

Peptide Inhibition Assay: Target band detection in rat skin tissue lysate was prevented by pre-blocking of a representative lot of this antibody with rat tail collagen type I.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Collagen Type I in rat skin tissue lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Evaluated by Immunohistochemistry (Paraffin) in rat skin tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Collagen Type I in rat skin tissue sections.

Control
Rat skin tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB755P

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.