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Merck

MAB4444

Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8

clone mab 8, from mouse

Synonym(s):

Adenocarcinoma-associated antigen, CD326 antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2, antigen identified by monoclonal antibo

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
mab 8, monoclonal
Application:
flow cytometry
immunocytochemistry
western blot
Species reactivity:
human
Citations:
Technique(s):
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
Uniprot accession no.:
P16422

Key Documents

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Product Name

Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8, clone mab 8, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

mab 8, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_002345.2

UniProt accession no.

P16422

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Application

Research Category
Stem Cell Research
Flow Cytometry Analysis: 0.5 µg from a previous lot detected Epithelial cell adhesion molecule in human embryonic stem cells.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Ng, V., et al., 2010)
Research Sub Category
Pluripotent & Early Differentiation

Adhesion (CAMs)
This Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).

Analysis Note

Control
Human embryonic stem cells
Evaluated by Immunocytochemistry in human embryonic stem cells.

Immunocytochemistry Analysis: 1-10 µg/mL from a previous lot detected epithelial cell adhesion molecule (EpCAM) in human embryonic stem cells.

Biochem/physiol Actions

This antibody recognizes epithelial cell adhesion molecule (EpCAM), specifically on undifferentiated human embryonic stem cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epithelial Call Adhesion Molecule (EpCAM, CD326) is a transmembrane glycoprotein of approximately 40 kDa. EpCAM was initially identified as a tumor associated marker having high expression in quickly proliferating epithelial tumors. As such, EpCAM has been of significant interest as a means for tumor diagnosis and therapy. EpCAM is also expressed in normal epithelial cells, but in comparatively lower levels. Preliminary studies limited EpCAM’s function to cell-cell adhesion; however more recent studies indicate it to be involved in diverse processes that include cell migration, cell signaling, differentiation and proliferation.
~ 40 kDa

Immunogen

Epitope: Unknown
HES-3 Cells (Choo, et al. (2008) Stem Cells. Jun;26(6):1454-63)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB4444:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MAB444404053252617447

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