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Merck

MABC965

Anti-Prohibitin-1 (PHB1), clone 1G5C1 Antibody

clone 1G5C1, from rat

Synonym(s):

Prohibitin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1G5C1, monoclonal
Application:
IHC, WB
Citations:
-

Key Documents

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biological source

rat

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G5C1, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001268425

UniProt accession no.

P35232

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PHB(5245)

General description

Prohibitin is an inhibitor of DNA synthesis that is widely expressed across various tissues. Prohibitin influences proliferation, and may also have a role in regulating mitochondrial respiration activity and in aging. Expression levels of prohibitin in fibroblasts have been found to decrease heterogeneously as cellular aging occurs.
~30 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Prohibitin-1 (PHB1).

Application

Anti-Prohibitin-1 (PHB1), clone 1G5C1 | MABC965 is an antibody against Prohibitin-1 for use in Western Blotting, Immunohistochemistry.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Prohibitin-1 (PHB1) in 10 µg of NIH/3T3 and HeLa cell lysate.
Immunohistochemistry Analysis: A representative lot detected Prohibitin-1 (PHB1) in rat liver tissue (Kakehashi, A., et al. (2011). Toxicological Sciences. 119(1):61-72).

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in rat liver tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Prohibitin-1 (PHB1) in 10 µg of rat liver tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABC965:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABC96504055977125290

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