AB5118
Anti-Cyclooxygenase-2 Antibody
Chemicon®, from rabbit
Synonym(s):
COX-2, PGHS-2, PHS-2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, WB
Citations:
9
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
bovine, sheep, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PTGS2(5743)
Immunogen
17 amino acid synthetic peptide corresponding to a sequence near the C-terminus of Human/Mouse/Rat COX-2 coupled to KLH.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Neuroinflammation & Pain
This Anti-Cyclooxygenase-2 Antibody is validated for use in ELISA, WB for the detection of Cyclooxygenase-2.
Western blot: 1-10 μg/mL using Chemiluminescence technique; COX-2 runs as a 68-72KDa protein in most cells, sometimes a 90kDa post-translationally modified form is seen as well. CaCo-2 line extracts (induced with serum or growth factors) are good positive controls.
ELISA: 1:10,000 - 1:100,000 using 100 ng of immunogen peptide per well.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000 - 1:100,000 using 100 ng of immunogen peptide per well.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Cyclooxygenase-2. Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 and COX2. Both forms of COX proteins are membrane associated heme proteins containing cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin. COX2 is a 72 kDa protein having 60% homology to COX1. It is induced by cytokines and mitogens and likely plays a role in inflammatory diseases such as rheumatoid arthritis.
Physical form
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
AB5118:
jan
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Qiong Cao et al.
PloS one, 12(10), e0186764-e0186764 (2017-10-20)
Microglia activation and associated inflammatory response are involved in the pathogenesis of different neurodegenerative diseases. We have reported that Notch-1 and NF-κB/p65 signalling pathways operate in synergy in regulating the production of proinflammatory mediators in activated microglia. In the latter
Clark A Lindgren et al.
The Journal of physiology, 591(19), 4749-4764 (2013-07-03)
Previous work has demonstrated that activation of muscarinic acetylcholine receptors at the lizard neuromuscular junction (NMJ) induces a biphasic modulation of evoked neurotransmitter release: an initial depression followed by a delayed enhancement. The depression is mediated by the release of
Marta Di Federico et al.
Frontiers in veterinary science, 7, 142-142 (2020-04-16)
Mycoplasma mycoides subsp. mycoides (Mmm) is the etiological agent of contagious bovine pleuropneumonia (CBPP), one of the major diseases affecting cattle in sub-Saharan Africa. Some evidences suggest that the immune system of the host (cattle) plays an important role in
Mark R Griffiths et al.
Immunology, 155(3), 346-355 (2018-06-21)
Microglia and non-professional immune cells (endothelial cells, neurons) participate in the recognition and removal of pathogens and tissue debris in the injured central nervous system through major pro-inflammatory processes. However, the mechanisms involved in regulating these responses remain ill-characterized. We
Ju-Yong Song et al.
Pharmacology, 102(3-4), 142-153 (2018-07-18)
Previous studies have suggested that quercetin is effective for treating diverse chronic disorders including organ fibrosis and airway and cardiovascular disorders. To access the pharmacological background for its broad efficacy, we examined the ability of quercetin to modulate the inflammatory
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB5118 | 04053252737985 |
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