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AB758

Anti-Collagen Type I (COL1A1) Antibody

CHEMICON®, goat polyclonal

Synonym(s):

Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human, bovine
Application:
DB, ELISA, ICC, IHC, WB
Citations:
30
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Product Name

Anti-Collagen Type I Antibody, Chemicon®, from goat

Quality Segment

100

biological source

goat

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NP_000079

UniProt accession no.

P02452

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

Immunogen

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

Application

Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.
Dot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Physical form

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360FD

Hazard Classifications

Repr. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

AB758:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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