Skip to Content

Key Documents

View All Documentation

CC130

Human Vitronectin

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM®

Synonym(s):

S-protein, Serum-spreading factor, V75

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
Escherichia coli, human
Form:
liquid
Technique(s):
cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable
Concentration:
0.5 mg/mL
Assay:
≥92% (SDS-PAGE)
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

PluriSTEM-XF® Recombinant Vitronectin, Human pluripotent stem cell culture.

Quality Segment

100

biological source

Escherichia coli, human

assay

≥92% (SDS-PAGE)

form

liquid

manufacturer/tradename

PluriSTEM®

concentration

0.5 mg/mL

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

input

sample type: human embryonic stem cell(s)

UniProt accession no.

P04004

shipped in

dry ice

storage temp.

-10 to -25°C

General description

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Application

Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion
Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Physical form

Product is supplied in a 20 mM pH 8.0 Tris-HCl buffer with a proprietary formulation of sucrose, EDTA, arginine, and DTT.

Preparation Note

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. Product has been 0.2 μm filtered.

Analysis Note

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under PluriSTEM-XF® Recombinant Vitronectin

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

CC130:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library