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Merck

CDUFBI0A1

Biopak® Polisher

Ultrafilter that removes bacteria, pyrogens, nucleases, and proteases from pure and ultrapure water at the point of dispense of Milli-Q® IQ/IX/EQ systems.

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About This Item

UNSPSC Code:
41104212
NACRES:
JA.13
eCl@ss:
33050190
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material

polysulfone filter

packaging

pkg of 1 unit

manufacturer/tradename

BioPak®

technique(s)

ELISA: suitable, PCR: suitable, cell culture | mammalian: suitable

compatibility

for use with Milli-Q® EQ 7000, for use with Milli-Q® EQ 7008, for use with Milli-Q® EQ 7016, for use with Milli-Q® IX 7003, for use with Milli-Q® IX 7005, for use with Milli-Q® IX 7010, for use with Milli-Q® IX 7015, for use with Milli-Q® IQ 7000, for use with Milli-Q® IQ 7003, for use with Milli-Q® IQ 7005, for use with Milli-Q® IQ 7010, for use with Milli-Q® IQ 7015

shipped in

ambient

Quality Level

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General description

The Biopak® Polisher is an ultrafiltration cartridge designed to produce water suitable for molecular biology, biochemistry and cell culture when fed with Milli-Q® purified water. It is validated for continuous use for 90 days.

Application

Biopak® Polisher ultrafiltration cartridge is suitable for use with Milli-Q® IX 7003/05/10/15, Milli-Q® IQ 7000, Milli-Q® IQ 7003/05/10/15, Milli-Q® EQ 7000 and Milli-Q® EQ 7008/16 water purification systems.. Designed for applications such as molecular biology, biochemistry and cell culture (when fed with Milli-Q® purified water), the Biopak® ultrafiltration unit removes macromolecules and larger biological structures, such as bacteria.

Biopak® Polisher is recommended for a wide range of laboratory applications where bacteria, pyrogen, nuclease and/or protease levels must be controlled.
This includes small-interfering RNA (siRNA) research, RNA in situ hybridization, microarray development, or studies of protein synthesis machinery.

Features and Benefits

  • Minimizes risk of nuclease, protease, pyrogen or bacteria contamination.
  • Eliminates the need for toxic agents such as DEPC, therefore improving safety, saving time and reducing costs.
  • Bottom-tip is redesigned for a secured installation of the protection bell.
  • Easy maintenance: the polisher is easily installed and replaced; does not require sanitization.
  • e-Sure tag for RFID connection with POD enables full traceability (data management) and automatic consumable status monitoring on the POD′s touchscreen interface.

Other Notes

Directions For Use
Organism Retention: Bacteria and pyrogens
Mode of Action: Ultrafiltration
Application: Cell culture media, molecular biology
Intended Use: Bioburden reduction
Instructions for Use: This item provides water filtered through ultrafiltration hollow fibers. Refer to insert, section "replacement" in the product package.
Storage Statement: Store in dry location.
Disposal Statement: Dispose of in accordance with applicable federal, state and local regulations.
  • Pyrogens (endotoxins) < 0.001 EU/mL
  • RNases < 1 pg/mL
  • DNases < 5 pg/mL
  • Protease < 0.15 μg/mL
  • Bacteria < 0.01 CFU/mL

Legal Information

BIOPAK is a registered trademark of Merck KGaA, Darmstadt, Germany
Milli-Q is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Real-time assay for testing components of protein synthesis
Rosenblum G, et al.
Nucleic Acids Research, 40(12), e88-e88 (2012)
Andrea Bleckmann et al.
Methods in molecular biology (Clifton, N.J.), 1669, 159-171 (2017-09-25)
A key element to understand developmental and reproductive processes like germline development, double fertilization, and embryogenesis is the study of cell-specific gene expression patterns which is best analyzed by RNA in situ hybridization. Different visualization techniques have been established to
Gabriel Rosenblum et al.
Nucleic acids research, 40(12), e88-e88 (2012-03-17)
We present a flexible, real-time-coupled transcription-translation assay that involves the continuous monitoring of fluorescent Emerald GFP formation. Along with numerical simulation of a reaction kinetics model, the assay permits quantitative estimation of the effects on full-length protein synthesis of various
Graphene oxide size and oxidation degree govern its supramolecular interactions with siRNA
Reina G, et al.
Nanoscale, 10(30), 5965-5974 (2018)
Detection and Quantification of MicroRNAs by Ligase-Assisted Sandwich Hybridization on a Microarray
Iizuka R, et al.
Microarrays, 1368 (2016)

Articles

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.

Ultrapure water from Milli-Q® system shown free of BPA, suitable for embryology research, minimizing endocrine disruptor interference.

See All

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Electrophysiology assays: Importance of ultrapure water and Biopak® cartridge filtration to avoid contamination.

Global Trade Item Number

SKUGTIN
CDUFBI0A104054839323461

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