MILLIPLEX^® Human Immuno-Oncology Checkpoint Protein Panel

Immune checkpoint proteins, such as co-inhibitory molecules (e.g., IDO1, B7-H3) and co-stimulatory signals (e.g., OX40, 4-1BB), orchestrate immune activation and tolerance, playing dual roles in tumor immune evasion and therapeutic response. While inhibitors targeting CTLA-4 and PD-1/PD-L1 have revolutionized cancer treatment, resistance and variable patient outcomes underscore the need to explore novel checkpoints (VISTA, CD73) and tumor microenvironment mediators (Galectin-3, Arginase-1). Emerging research highlights soluble isoforms and lesser-studied membrane proteins as predictive biomarkers and therapeutic targets, driving efforts to decode their spatial, temporal, and combinatorial roles in immune suppression. This multifaceted landscape demands high-dimensional profiling to unravel mechanisms of resistance, optimize combination therapies, and identify sample subsets poised to benefit from next-gen immunotherapies.

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 31 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: 4-1BBL/TNFSF9, 5′-NT/CD73 (5′-nucleotidase), APRIL (TNFSF13), Arginase-1, B7-H2/ICOSL (Inducible T cell costimulator ligand), B7-H3/CD276, B7-H4/VTCN1 (V-set domain-containing T-cell activation inhibitor 1), B7-H5/VISTA (V-set immunoregulatory receptor), B7-H6 (Natural killer cell cytotoxicity receptor 3 ligand), BAFF/BLyS (TNFSF13B), CD25/IL-2Rα (Interleukin-2 receptor alpha), CD30/TNFRSF8, CD40L (CD40 ligand), CD137/4-1BB (TNFRSF9), CD226/DNAM-1 (DNAX accessory molecule 1), E-Cadherin, FGL1/Hepassocin (Fibrinogen-like protein 1), Galectin-1 (Gal-1), Galectin-3 (Gal-3), Granulysin, Granzyme B, IDO1 (Indoleamine 2,3-dioxygenase 1), MICA (MHC class I polypeptide-related sequence A), MICB (MHC class I polypeptide-related sequence B), Nectin-2 (PVRL2, CD112), Nectin-4 (PVRL4), OX40/CD134 (TNFRSF4), Perforin, PVR/CD155 (Poliovirus receptor cell adhesion molecule), Siglec-7 (Sialic acid-binding Ig-like lectin 7, CD328), Siglec-9 (Sialic acid-binding Ig-like lectin 9, CD329)

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Luminex is a registered trademark of Luminex Corporation

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

For research use only. Not for use in diagnostic procedures.

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By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.