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MAB1393Z

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free

clone TLD-3A12, Chemicon®, from mouse

Synonym(s):

CD31, MAB1393

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
TLD-3A12, monoclonal
Application:
ELISA, FACS, IHC, WB
Citations:
4
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

TLD-3A12, monoclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

UniProt accession no.

Q3SWT0

shipped in

dry ice

target post-translational modification

unmodified

Immunogen

Activated, Lewis rat derived microglial cells.

Application

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free detects level of PECAM-1 & has been published & validated for use in ELISA, FC, WB, IH.
Flow Cytometry: 1:50-1:100

Immunohistology: frozen sections (-20°C methanol fixation), not recommended for traditional formalin fixed tissues.

ELISA

Western Blotting: 1:500. MW = 130-140 kDa

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Biochem/physiol Actions

Rat PECAM-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1, confirming specificity.

Physical form

Format: Purified
Purified immunoglobulin by affinity chromatography on Protein G. Liquid in phosphate buffered saline containing no preservatives.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: Activated microglial cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB1393Z:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yingchao Shi et al.
PLoS biology, 18(5), e3000705-e3000705 (2020-05-14)
Modeling the processes of neuronal progenitor proliferation and differentiation to produce mature cortical neuron subtypes is essential for the study of human brain development and the search for potential cell therapies. We demonstrated a novel paradigm for the generation of

Global Trade Item Number

SKUGTIN
MAB1393Z04053252505652