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MAB2575

Anti-Integrin alpha 5 (ITGA5/CD49E) Antibody

CHEMICON®, rat monoclonal, BMC5

Synonym(s):

VLA-5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
BMC5, monoclonal
Species reactivity:
mouse
Application:
FACS, IHC, IP
Citations:
5
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Product Name

Anti-Integrin α5β1 Antibody, clone BMC5, ascites fluid, clone BMC5, Chemicon®

biological source

rat

Quality Segment

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BMC5, monoclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG2b

NCBI accession no.

NM_002205.2

UniProt accession no.

P08648

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ITGA5(3678)

Immunogen

Mouse embryonal carcinoma P19 cells.

Application

Detect Integrin α5β1 using this Anti-Integrin α5β1 Antibody, clone BMC5 validated for use in FC, IP, IH.
Inhibition of cell adhesion to fibronectin: 10 μL/mL

Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.

Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL

Flow cytometry: 10μL/mL

Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Biochem/physiol Actions

Antibody MAB2575 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.

Physical form

Liquid ascites.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB2575:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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