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MAB463

Anti-Estrogen Receptor Antibody, clone B10

ascites fluid, clone B10, Chemicon®

Synonym(s):

ER

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
B10, monoclonal
Application:
ELISA, IHC, WB
Citations:
9
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

B10, monoclonal

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG2a

NCBI accession no.

NP_000116.2

UniProt accession no.

P03372

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HSPG2(3339)

Immunogen

Epitope: a.a. 151-165

Application

Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000

Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000

ELISA at 1:1,000-1:2,000

Western blot analysis at 1:1,000-1:2,000

Immunoprecipitation at 1:1-1:10

Gel super shifts at 1:1-1:10

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.

Biochem/physiol Actions

The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.

SPECIES REACTIVITIES:

Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.

Physical form

Ascites. Liquid, does not contain any preservative.

Preparation Note

Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB463:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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