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MABT523

Anti-β-Actin (ACTB) Antibody

rabbit monoclonal, RM112

Synonym(s):

Actin, cytoplasmic 1, Beta-actin, beta-Actin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
RM112, monoclonal
Species reactivity:
human
Application:
ICC, WB
Citations:
22
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Product Name

Anti-beta-Actin Antibody, clone RM112, clone RM112, from rabbit

biological source

rabbit

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

RM112, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable, western blot: suitable

isotype

IgG

UniProt accession no.

P60709

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

General description

Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunogen

A peptide corresponding to the N-terminus of beta-Actin.

Application

Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.

This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABT523:

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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