3dGRO^® Human Liver Organoids (ID CS235474)

Patient derived organoids (PDO) are tissue derived, novel in vitro 3D cell models that recapitulate some key features of the respective organ, thus representing a more in vivo-like alternative to traditional 2D cell cultures. In addition, PDOs offer possible means to reduce animal model use. PDO maybe derived from resections or biopsy of adult tissue and are phenotypically and genetically stable in culture. PDO are an ideal platform for drug screening, disease modeling and tumor immunity studies, and may also be modified by gene editing.

The 3dGRO^® human liver organoid biobank is a collection of tissue-derived normal non-diseased human liver organoids from numerous patients. The biobank contains liver PDOs that are highly viable and express high levels of albumin, HNF4-a, FOXA2, SOX17, CK7, CK19, E-Cadherin, PCK2 along with important cytochrome P450 (CYP) enzymes activities (Cyp3A4, Cyp2C9, Cyp2B6, Cyp2D6, Cyp1A2).

Cell Line Characteristics

• Sex: Female
• Age: 57 Years
• Organ: Liver
• Disease: Normal
• Medications: Advil, Tylenol, Zosyn
• Patient History: COD: Anoxia, BMI: 30.27, Alcohol: binge drinking 3d/week X 10 yrs; Tobacco: 1 pack every 3-4 days X 3 yrs; Drugs: Methamphetamine 2d/week X 3 yrs. Used Fentanyl

Cryopreserved patient derived human liver organoids (PDOs)

Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

3dGRO^® is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO^® organoids were derived utilizing HUB Organoid Technology.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.