SCR128
Dopaminergic Differentiation Growth Factor Sampler
This Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
eCl@ss:
32160405
Quality Segment
assay
>98% (SDS-PAGE and HPLC)
impurities
<0.1 ng/μg endotoxin (1EU/μg)
shipped in
dry ice
storage temp.
-10 to -25°C
General description
EMD Millipore’s Dopaminergic Differentiation Growth Factor Sampler contains a collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons along with a reliable high affinity antibody marker, anti-tyrosine hydrodylase, to aid in quantifying the percentage yields from differentiation experiments.
Product Source: E. coli.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
This Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.
Biochem/physiol Actions
Cross Reactivty
None
None
Preparation Note
Store at -20°C for up to 6 months from date of receipt.
Analysis Note
Using the growth factors supplied in this kit a 3-5 fold increase of dopaminergic differention (up to 50-70% TH+ cells) was routinely produced using HES/hips cell derived neural progenitor cells vs. spontaneous neuronal differentiation.
Other Notes
1. Sonic HedgeHog (Shh), Human Recombinant (Part No. GF174-5UG): Two 5 μg vials. Lyophilized powder. Store at -20°C.
2. Fibroblast Growth Factor-8 (FGF-8), Human Recombinant (Part No. GF110-5UG): One 5 μg vial. Lyophilized powder. Store at -20°C.
3. Brain Derived Neurotrophic Factor (BDNF), Human Recombinant (Part No. GF029-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
4. Glial Derived Neurotrophic Factor (GDNF), Human Recombinant (Part No. GF030-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
5. Transforming Growth Factor β-III (TGF-β-III), Human Recombinant (Part No. GF176-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
6. Anti-Tyrosine Hydroxylase antibody, rabbit polyclonal (Part No. AB152-20): One 20 μL vial. Store at -20°C.
2. Fibroblast Growth Factor-8 (FGF-8), Human Recombinant (Part No. GF110-5UG): One 5 μg vial. Lyophilized powder. Store at -20°C.
3. Brain Derived Neurotrophic Factor (BDNF), Human Recombinant (Part No. GF029-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
4. Glial Derived Neurotrophic Factor (GDNF), Human Recombinant (Part No. GF030-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
5. Transforming Growth Factor β-III (TGF-β-III), Human Recombinant (Part No. GF176-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
6. Anti-Tyrosine Hydroxylase antibody, rabbit polyclonal (Part No. AB152-20): One 20 μL vial. Store at -20°C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 1
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Please refer to KIT Component information
pdsc
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prtr
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fsl
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ishl_indicated
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ishl_notified
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Certificates of Analysis (COA)
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