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Merck

251760

DAR-4M

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

Synonym(s):

DAR-4M, Diaminorhodamine-4M

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About This Item

Empirical Formula (Hill Notation):
C25H26N4O3
Molecular Weight:
430.50
NACRES:
NA.25
UNSPSC Code:
12352202
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Product Name

DAR-4M, A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

red-violet

fluorescence

λex ~560 nm
λem ~575 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

Biochem/physiol Actions

Cell permeable: no
Primary Target
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Irritant (B)

Legal Information

Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Physical form

In DMSO.

Preparation Note

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

General description

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500 ).

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C


Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
251760-1MGCN04055977217254

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