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Merck

405210

ID-8

A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

Synonym(s):

ID-8, 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol

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About This Item

Empirical Formula (Hill Notation):
C16H14N2O4
CAS Number:
147591-46-6
Molecular Weight:
298.29
MDL number:
MFCD00593367
UNSPSC Code:
12352200
NACRES:
NA.51

Key Documents

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Product Name

ID-8, A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

Quality Level

100

InChI key

VVZNWYXIOADGSW-UHFFFAOYSA-N

InChI

1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3

SMILES string

[N+](=O)([O-])c1c2c([n](c1C)c3ccc(cc3)OC)cc(cc2)O

assay

≥95% (HPLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 50 mg/mL

shipped in

ambient

storage temp.

2-8°C

Disclaimer

Toxicity: Standard Handling (A)

General description

A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.

Other Notes

Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.

Packaging

Packaged under inert gas

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

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Tomoyuki Miyabayashi et al.
Bioscience, biotechnology, and biochemistry, 72(5), 1242-1248 (2008-05-08)
Embryonic stem cells (ESCs), which have characteristics such as self-renewal, indefinite proliferation, and pluripotency, are thought to hold great promise for regenerative medicine. ESCs are generally cultured on mouse embryonic fibroblast (MEF) or MEF conditioned medium (MEF-CM). However, for therapeutic

Global Trade Item Number

SKUGTIN
405210-10MGCN04055977211856

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