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Merck

567333

SNAPtide Botulinum Toxin A Substrate, Fluorogenic

≥90% (HPLC), lyophilized, FRET substrate, Calbiochem®

Synonym(s):

Botulinum Toxin Assay

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

Key Documents

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Product Name

SNAPtide Botulinum Toxin A Substrate, Fluorogenic, SNAPtide Botulinum Toxin A Substrate, Fluorogenic, is a synthetic peptide that acts as a FRET substrate with the fluorophore o-aminobenzoic acid and the acceptor chromophore 2,4-dinitrophenol (DNP).

assay

≥90% (HPLC)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

fluorescence

λex ~326 nm
λem ~423 nm

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate for the native botulinum toxin
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

A synthetic peptide, fluorescence resonance energy transfer (FRET) substrate containing the N-terminally-linked fluorophore o-aminobenzoic acid (Abz) and the acceptor chromophore 2,4-dinitrophenol (DNP) linked to the C-terminus. The substrate is based on the native botulinum toxin type A cleavage site found in the synaptosome-associated protein SNAP-25. Cleavage of the substrate by botulinum toxin releases the fluorophore and full fluorescence is restored.

Other Notes

Bigalke, H. and Shoer, L.F. 2000. In: Handbook of Experimental Pharmacology,145, Bacterial Protein Toxins (K. Aktories and I. Just, Eds.) pp. 407-443, Springer-Verlag, Berlin.
Schiavo, G., et al. 1993. J. Biol. Chem.268, 23784.

Physical form

Lyophilized from water.

Preparation Note

Following reconstitution, aliquot, cover with foil to protect from light, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Prepare a 5 mM stock solution of this peptide in DMSO as follows: Add 40 µl of DMSO to a vial containing 200 nmol of peptide.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
567333-200NMOLCN04055977267211

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