DC03L
Goat Anti-Rabbit IgG Peroxidase Conjugate
lyophilized, Calbiochem®
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
rabbit
Application:
—
Citations:
36
biological source
goat
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
rabbit
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (1:5000-1:100,000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000))
isotype
IgG
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Related Categories
General description
Immunoaffinity purified goat polyclonal antibody adsorbed against human serum proteins and conjugated to horseradish peroxidase. Recognizes rabbit IgG.
This Goat Anti-Rabbit IgG Peroxidase Conjugate is validated for use in ELISA, Immunohistochemistry for the detection of Rabbit IgG.
Application
ELISA (1:5000-1:100,000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000)
Physical form
Lyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.
Preparation Note
Reconstitute the antibody with 1.5 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.
Other Notes
To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for rabbit IgG and light chains common to most rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
11 - Combustible Solids
wgk
WGK 3
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
DC03L-MG: + DC03L-200UG:
jan
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Karl A Nath et al.
The American journal of pathology, 166(4), 963-972 (2005-03-29)
Ischemic injury is invoked as a mechanism contributing to end-organ damage and other complications of sickle cell disease (SCD). However, the intrinsic sensitivity of tissues in SCD to ischemic insults has never been addressed. We examined the effect of renal
Katherine A Blackwell et al.
American journal of physiology. Heart and circulatory physiology, 287(6), H2448-H2453 (2004-08-21)
Oxidative stress has been implicated as an important mechanism of vascular endothelial dysfunction induced by aging. Previous studies suggested that tetrahydrobiopterin (BH4), an essential cofactor of endothelial NO synthase, could be a molecular target for oxidation. We tested the hypothesis
K Tello et al.
Radiation and environmental biophysics, 47(3), 389-397 (2008-05-22)
In a previous publication, we were able to show that irradiation of Kupffer cells, the liver resident macrophages, leads to an increased TNF-alpha concentration in the culture medium. The pathomechanisms underlying this phenomenon, however, remained to be elucidated. Here, we
Emmanuelle Thinon et al.
Journal of proteome research, 17(5), 1907-1922 (2018-03-27)
S-Fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryotes and is important
Bettina P Mihalas et al.
Aging, 11(10), 2968-2997 (2019-05-28)
Oocytes are reliant on messenger RNA (mRNA) stores to support their survival and integrity during a protracted period of transcriptional dormancy as they await ovulation. Oocytes are, however, known to experience an age-associated alteration in mRNA transcript abundance, a phenomenon
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| DC03L-200UGCN | 07790788053727 |
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