SCR011
B6-White Murine ES Cell Line
The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function.
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Product Name
B6-White Murine ES Cell Line, The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function.
biological source
mouse
manufacturer/tradename
B6-White
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
shipped in
liquid nitrogen
Quality Level
Application
Stem Cell Research
Biochem/physiol Actions
Disclaimer
General description
Millipore′s B6-White Murine ES cell line is the first commercially available C57BL/6 tyrc-2J albino line that allows for rapid coat-color determination of successful chimerism in the C57BL/6 mouse strain. When B6-White ES cells are injected into C57BL/6 blastocysts, chimeric mice are easily identified by their coat color (a mix of black and white patches), whilst non-chimeric littermates are black. These cells allow for the efficient generation of gene-targeted mice in a pure C57BL/6 genetic background, thus providing more experimental flexibility. Additionally the use of these cells can lower production costs by eliminating the need to maintain an albino blast donor colony, in order to assess chimerism by coat color when targeting in the C57BL/6 strain.
For germline transmission confirmation by coat color, it is recommended that male chimeras are mated with homozygous females of the B6-white strain (strain name: C57BL/6J-Tyrc-2J/J - available from Jackson Labs, stock number 000058).
STRAIN:C57BL/6-tyr(c-2J) albino mice
GENDER: Male
KARYOTYPE: 40XY
Physical form
Preparation Note
Storage Class
10 - Combustible liquids
wgk
WGK 2
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Related Content
The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.
"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCR011 | 04053252317941 |
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