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Merck

A7906

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
9048-46-8
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
MFCD00130384
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
blood typing: suitable, microbiological culture: suitable
Impurities:
≤5.0% Loss on drying

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biological source

bovine

assay

≥98%

form

lyophilized powder

Quality Level

300

Gene Information

bovine ... ALB(280717)

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blood typing: suitable, microbiological culture: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT virus, none detected, VSV virus, none detected

storage temp.

2-8°C

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Application

Bovine serum albumin has been used-
  • as a component of PCR (polymerase chain reaction) mix
  • in the preparation of dissociated cortical neurons
  • during immunofluorescent labeling
  • as a component of Ca2+-free saline (containing collagenase, hyaluronidase and DNAse) used for bovine tissue digestion

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

A7906-1KG: + A7906-50G: + A7906-5G: + A7906-10KG: + A7906-5KG: + A7906-VAR: + A7906-500G: + A7906-6X100G: + A7906-250G: + A7906-10G: + A7906-100G: + A7906-BULK: + A7906-BULK-NX:

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Certificates of Analysis (COA)

Lot/Batch Number
0000472701
0000472557
0000472701
0000472557
0000472287

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V Templier et al.
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Maconochie DJ and Knight DE
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New tetranucleotide microsatellites for fine-scale discrimination among endangered Chinook salmon (Oncorhynchus tshawytscha).
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