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S2002

Sodium azide

ReagentPlus®, ≥99.5%

Synonym(s):

Hydrazoic acid sodium salt

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About This Item

Linear Formula:
NaN3
CAS Number:
26628-22-8
Molecular Weight:
65.01
NACRES:
NA.21
PubChem Substance ID:
24899504
UNSPSC Code:
12352201
EC Number:
247-852-1
MDL number:
MFCD00003536
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biological source

synthetic (inorganic)

Quality Level

200

product line

ReagentPlus®

assay

≥99.5%

form

powder

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

technique(s)

cell based assay: suitable, immunohistochemistry: suitable

color

white

mp

370-425 °C

solubility

soluble 65 g/L at 20 °C (completely)

suitability

suitable for chromatography, suitable for derivatization

application(s)

sample preparation

SMILES string

[Na]N=[N+]=[N-]

InChI

1S/N3.Na/c1-3-2;/q-1;+1

InChI key

PXIPVTKHYLBLMZ-UHFFFAOYSA-N

General description

Sodium Azide (NaN3) is a potent inhibitor – a water-soluble crystalline powder that′s odorless. Widely recognized for its versatile applications, it plays a pivotal role across molecular biology, cell culture, and biochemical research. It is also a metabolic inhibitor, which inhibits oxidative phosphorylation. In cell culture, it maintains the integrity of your cell lines.

Application

Sodium azide is suitable for use in histopathology to prepare and store tissue samples. Also used as a component of staining buffer (HEPES buffer) for whole mount immunolabelling.
Catalyst for:

  • Oxidative decarboxylation
  • Michael addition reactions

Reagent for synthesis of
  • Blue fluorescent copolymers
  • Metal phosphonates
  • Arenes via aminations
Baylis-Hillman acetates such as ethyl 2-(acetoxyphenylmethyl)acrylate can undergo nucleophilic displacement by sodium azide in aqueous medium to form ethyl (E)-2-azidomethyl-3-phenylpropenoate.

Biochem/physiol Actions

It prevents capping and internalization of fluorescent surface-bound antibodies in various scientific applications, including flow cytometry.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2 Oral

target_organs

Brain

supp_hazards

EUH032

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Poisonous substance

pdsc

Group 5: Self-reactive substances + Metal azides + Hazardous rank II + 2nd self-reactive materials

fsl

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

S2002-5G:4.548173317236E12 + S2002-25G:4.548173210131E12 + S2002-100G:4.548173210124E12 + S2002-2KG:4.548173210148E12 + S2002-500G:4.548173210155E12

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP6472
MKDB2154
MKCQ1781
BCCN8988
BCCN8989

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Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Antibody Purification using Protein A/G/L Agarose

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

Calcium uptake by rat kidney mitochondria.
DeLuca HF and Engstrom GW.
Proceedings of the National Academy of Sciences of the USA, 47(11), 1744-1750 (1961)
A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination
Patricia A Arndt , George Garratty
Transfusion Medicine Reviews, 24(3), 174-192 (2010)
Sodium azide, a bacteriostatic preservative contained in commercially available laboratory reagents, influences the responses of human platelets via the cGMP/PKG/VASP pathway.
Russo I, et al.
Clinical Biochemistry, 41(4), 343-349 (2008)
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