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01494

Nancy-520

Name: Nancy-520
Brand: SIGMA

suitable for RT-PCR, ≥90.0% (HPCE)

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About This Item

UNSPSC Code:

12171500

EC Number:

200-664-3

NACRES:

NA.32

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Properties

Quality Level

100

assay

≥90.0% (HPCE)

form

liquid

mol wt

78.13 g/mol

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

color

red to very dark red

bp

189 °C/1,013 hPa

mp

18.4 °C

solubility

DMSO: soluble

density

1.104 g/cm³ at 20 °C

fluorescence

λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA

greener alternative category

Aligned,

storage temp.

2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup - Solvent

flash_point_c

87 °C - closed cup - Solvent

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water soluble liquid

fsl

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Articles

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

定量PCRの基礎

サンプル中の出発物質の量の推定を可能にするリアルタイムPCRは、従来のPCRよりもはるかに広いダイナミックレンジを持ちます。