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59304

2-Propanol

≥99.5% (GC), for molecular biology, BioUltra

greener alternative

Greener Alternative

Synonym(s):

sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

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About This Item

Linear Formula:
(CH3)2CHOH
CAS Number:
67-63-0
Molecular Weight:
60.10
NACRES:
NA.25
PubChem Substance ID:
57651672
UNSPSC Code:
12352104
EC Number:
200-661-7
MDL number:
MFCD00011674
Beilstein/REAXYS Number:
635639
eCl@ss:
32150313
Assay:
≥99.5% (GC)
Grade:
Molecular Biology
Technique(s):
DNA extraction: suitable, RNA extraction: suitable
Bp:
82 °C (lit.)
Vapor pressure:
33 mmHg ( 20 °C), 44 mmHg ( 25 °C)
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Product Name

2-Propanol, BioUltra, Molecular Biology, ≥99.5% (GC)

grade

Molecular Biology

Quality Segment

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

product line

BioUltra

assay

≥99.5% (GC)

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

sustainability

Sustainability features

technique(s)

DNA extraction: suitable, RNA extraction: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

evapn. residue

≤0.001%

color

colorless

refractive index

n20/D 1.377 (lit.), n20/D 1.378

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

SMILES string

CC(C)O

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤0.5 mg/kg, Ni: ≤0.02 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.02, λ: 280 nm Amax: 0.01

suitability

suitable for molecular biology

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

General description

2-propanol is a clear, colorless, polar organic solvent commonly used in chemical and biochemical laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. Isopropanol is commonly used in molecular biology for DNA and RNA extraction through precipitation. Unlike ethanol, which requires 2.5 times the volume of the nucleic acid-containing sample, isopropanol can be added in an equal volume. This causes precipitation to occur even without cooling, making it advantageous in reducing the overall volume of the sample. Moreover, it has applications in quantifying hydroxyproline levels, assessing cell viability through MTT assays, and contributing to microarray hybridization processes.

Application

2-Propanol has been used:
  • to precipitate genomic DNA extracted from flies
  • to precipitate genomic DNA extracted from xenograft primary tumors and organs
  • to immunoprecipitate 5-bromo-2-deoxyuridine (BrdU)

Biochem/physiol Actions

Isopropanol (2-Propanol) is widely used in molecular biology for the precipitation of DNA during extraction from cells and tissues. BioUltra IPA is suitable for DNA precipitation.

Features and Benefits

This product aligns with one or more of the 12 Principles of Green ChemistryThis solvent is safe to use and thus aligns with "Safer Solvents and Auxiliaries". Click here for more information.
  • Suitable as a reagent for Biochemical and Molecular Biology Research
  • BioUltra grade, meets strict purity standards through rigorous testing
  • Free from DNase, NICKase, RNase, and Protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Codes

H225,H319,H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

53.6 °F - closed cup

Flash Point (°C)

12.0 °C - closed cup

PPE (personal protective equipment)

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Alcohols + Hazardous rank II

fsl

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

59304-100ML-F:4548173172460 + 59304-500ML-F:4548173172484 + 59304-6X1L-F:4548173270463 + 59304-6X500ML-F:4548173270470 + 59304-1L-F:4548173172477

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