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D4418

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Synonym(s):

DAB, DAB tablets, Immunohistology substrate

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About This Item

CAS Number:
91-95-2
UNSPSC Code:
12352204
PubChem Substance ID:
24893872
NACRES:
NA.83
MDL number:
MFCD00007725
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Product Name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 15 mL

Quality Segment

200

form

tablet

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Preparation Note

Each tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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Certificates of Analysis (COA)

Lot/Batch Number
0000523510
0000523511
0000523511
0000523510
0000484644

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Protocols

免疫組織染色のプロトコル

パラフィン包埋組織切片中の特定の抗原を染色および可視化する免疫組織染色（IHC）の全プロトコルをご紹介します。

ウェスタンブロッティングのための細胞溶解・タンパク質抽出

後続のウェスタンブロッティングのためのサンプル調製（培養組織・細胞の効果的な溶解とタンパク質抽出）プロトコル

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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