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L2542

LB Broth (Miller)

Liquid microbial growth medium

Synonym(s):

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

NACRES:
NA.85
UNSPSC Code:
41106200
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grade

Molecular Biology

Quality Segment

400

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.2

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Other Notes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

L2542-BULK: + L2542-1L: + L2542-VAR: + L2542PROC: + L2542-PH: + L2542-500ML:

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