Skip to Content
Merck

A4503

Bovine Serum Albumin

cold ethanol fraction, pH 5.2, ≥96%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
9048-46-8
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
MFCD00130384
Biological source:
bovine
Assay:
≥96%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
Impurities:
BT and VSV virus, tested

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

bovine

assay

≥96%

form

lyophilized powder

Quality Level

300

Gene Information

bovine ... ALB(280717)

purified by

cold ethanol fractionation

origin

USA origin

technique(s)

ELISA: suitable, blood typing: suitable, microbiological culture: suitable

impurities

BT and VSV virus, tested

loss

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected, VSV Virus, none detected

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

  • Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
  • It has been used in the cytotoxicity assay buffer.
  • It has been used for the induction of protein overload nephropathy in rats.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

A4503-100G: + A4503-VAR: + A4503-5G: + A4503-BULK: + A4503-10G: + A4503-50G: + A4503-1KG: + A4503-5KG: + A4503-500G: + A4503-10KG:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000529437
0000529436
0000529437
0000529436
0000523700

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Isabella Marcomini et al.
Cell reports, 24(10), 2614-2628 (2018-09-06)
Multiple pathways regulate the repair of double-strand breaks (DSBs) to suppress potentially dangerous ectopic recombination. Both sequence and chromatin context are thought to influence pathway choice between non-homologous end-joining (NHEJ) and homology-driven recombination. To test the effect of repetitive sequences
Cytochrome C effect on respiration of heart mitochondria: influence of various factors.
Toleikis A et al.
Bioscience Reports, 25, 387-387 (2005)
Sensitization of tumor cells to NK cell-mediated killing by proteasome inhibition.
Hallett WH et al.
Journal of Immunology, 180, 163-163 (2008)
Rui Zhang et al.
PLoS pathogens, 15(2), e1007329-e1007329 (2019-03-01)
Mycobacterial pathogens are the causative agents of chronic infectious diseases like tuberculosis and leprosy. Autophagy has recently emerged as an innate mechanism for defense against these intracellular pathogens. In vitro studies have shown that mycobacteria escaping from phagosomes into the
Simona Ranallo et al.
Nature communications, 8, 15150-15150 (2017-05-10)
A wide range of molecular devices with nanoscale dimensions have been recently designed to perform a variety of functions in response to specific molecular inputs. Only limited examples, however, utilize antibodies as regulatory inputs. In response to this, here we
View All Related Papers

Articles

Albumin in Cell Culture

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

Protocols

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

See All

Related Content

ウシ血清アルブミン　よくあるご質問（FAQ）

ウシ血清アルブミン（BSA）の構造、分子量、機能、溶解性、製造方法、精製方法、アプリケーションおよび購入に関する情報が得られます。

Albumin from Bovine Serum Frequently Asked Questions (FAQs)

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

Blood Proteins in Cell Culture

Discover why blood proteins such as bovine serum albumin, human serum albumin, globulins, and more are crucial for cell culture.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service