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Merck

A6964

Accutase® solution

sterile-filtered, suitable for cell culture

Synonym(s):

Cell detachment solution

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.75
MDL number:
MFCD01632905

Key Documents

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Product Name

Accutase® solution, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

400-600 units/mL

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

impurities

≤1 EU/mL endotoxin

pH

6.8-7.8

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Related Categories

Application

Accutase® solution has been used for dissociation or digestion of nonadherent cells growing in an aggregated cluster, such as brain tumor cells, induced pluripotent stem cells (IPSCs) and glioblastoma cells into single cells.

Suitable for use in preparation of single cell suspension for sequencing.
Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, and flow cytometry as well as bioreactor scale-up. Does not contain mammalian or bacterial-derived products.

Features and Benefits

Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware.

General description

Accutase® solution (in Dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion- coated plasticware. Accutase is preferred over trypsin for cell detachment as it helps to detach cells from culture vessels with less damage. Hence, Accutase is often used for the treatment of sensitive cells or for analyses of surface markers.

Preparation Note

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red. Refer to Product Data Sheet on the website for product usage information.
This product contains phenol red. The product is shipped on dry ice and there could be significant CO2 buildup in the package. This CO2 may enter the solution and lower the pH slightly giving a yellow-orange (around pH 6.8) color. The solution should still be good to use as is, or sodium hydroxide may be added to adjust the pH. A fully frozen bottle of Accutase will be yellow in color and change to an orange color as it defrosts. Once unpacked, the product can be refrozen or placed directly into the refrigerator for later use. Do not defrost the product in a warm water bath. Accutase is sensitive to temperatures above 37 °C and will be inactivated after 45 minutes at 37 °C. Defrosting overnight in the refrigerator or in a tub of cold tap water for 1-1.5 hours is recommended. Shake up the bottle of Accutase after defrosting to enable the ingredients to go into solution evenly. These products do not need to be warmed up before being used. Use promptly once removed from the refrigerator, and return to the refrigerator after use.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000450577
0000450577
0000470557
0000470551
0000470551

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Autophagy pathways in glioblastoma
Jiang H, et al.
Methods in Enzymology, 453, 273-286 (2009)
Yanting Xue et al.
Stem cell research, 24, 151-154 (2017-10-17)
Human MSX1 gene is mapped to chromosome 4 and encodes a 303aa homeobox protein MSX1. MSX1 expression appears during early tooth development of vertebrate embryogenesis. Mutations in this protein are related to human tooth anomalie, cleft lip and palate and
Frank-Peter Wachs et al.
Laboratory investigation; a journal of technical methods and pathology, 83(7), 949-962 (2003-07-16)
Neural stem cells (NSCs) from the adult central nervous system are currently being investigated for their potential use in autologous cell replacement strategies. High expansion rates of NSCs in culture are crucial for the generation of a sufficient amount of
Ruchi Bajpai et al.
Molecular reproduction and development, 75(5), 818-827 (2007-12-25)
Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency
Recent Zika virus isolates induce premature differentiation of neural progenitors in human brain organoids
Gabriel E, et al.
Cell Stem Cell, 20(3), 397-406 (2017)
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