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Merck

B7435

CelLytic™ B Cell Lysis Reagent

For bacterial cell lysis, standard strength

Synonym(s):

Cell lysis reagent

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About This Item

UNSPSC Code:
41116134
NACRES:
NA.77
MDL number:
MFCD02097909

Key Documents

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form

solution

Quality Level

200

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Application

A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
CelLytic™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.

Features and Benefits

  • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
  • Scalable for 1 to 25 grams of bacterial cell paste
  • No interference with downstream applications such as affinity chromatography, IP, and Western blotting
  • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

B7435-500ML: + B7435-BULK: + B7435-500ML-KC: + B7435-50ML-KC: + B7435-50ML: + B7435-500ML-EW: + B7435PROC: + B7435-VAR:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
0000262909
0000262909
0000485711
0000485711
0000467778

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Ken Motohashi
BMC biotechnology, 15, 47-47 (2015-06-04)
Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses the components of the λ prophage Red/ET recombination system. This method facilitates restriction endonuclease
Monika Raab et al.
Scientific reports, 9(1), 10462-10462 (2019-07-20)
While the immune cell adaptor protein SKAP1 mediates LFA-1 activation induced by antigen-receptor (TCR/CD3) ligation on T-cells, it is unclear whether the adaptor interacts with other mediators of T-cell function. In this context, the serine/threonine kinase, polo-like kinase (PLK1) regulates
Jozef Nahálka
Journal of industrial microbiology & biotechnology, 35(4), 219-223 (2007-12-19)
Maltodextrin phosphorylase from Pyrococcus furiosus (PF1535) was fused with the cellulose-binding domain of Clostridium cellulovorans serving as an aggregation module. After molecular cloning of the corresponding gene fusion construct and controlled expression in Escherichia coli BL21, 83% of total maltodextrin
Prion-like seeding of misfolded alpha-synuclein in the brains of dementia with Lewy body patients in RT-QUIC
Sano K, et al.
Molecular Neurobiology, 55(5), 3916-3930 (2018)
Kazunori Sano et al.
Acta neuropathologica communications, 9(1), 182-182 (2021-11-14)
Serine 129 (S129) phosphorylation of α-synuclein (αSyn) is a central feature of Lewy body (LB) disease pathology. Although the neighboring tyrosine residues Y125, Y133, and Y136 are also phosphorylation sites, little is known regarding potential roles of phosphorylation cross-talk between
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Product Information Sheet - B7435

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