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B7435

CelLytic™ B Cell Lysis Reagent

For bacterial cell lysis, standard strength

Synonym(s):

Cell lysis reagent

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About This Item

UNSPSC Code:
41116134
NACRES:
NA.77
MDL number:
MFCD02097909
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Quality Segment

form

solution

input

bacteria and yeast sample(s)

Application

CelLytic™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.

Features and Benefits

  • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
  • Scalable for 1 to 25 grams of bacterial cell paste
  • No interference with downstream applications such as affinity chromatography, IP, and Western blotting
  • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Other Notes

Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

B7435-500ML: + B7435-BULK: + B7435-500ML-KC: + B7435-50ML-KC: + B7435-50ML: + B7435-500ML-EW: + B7435PROC: + B7435-VAR:

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