CLLS1127
DLD1 Cells TP53 -/-
human male colon (Source Disease: Colorectal adenocarcinoma)
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human male colon (Source Disease: Colorectal adenocarcinoma)
Product Name
DLD1 Cells TP53 -/-,
biological source
human male colon (Source Disease: Colorectal adenocarcinoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... TP53(7157)
General description
DLD1 Cells TP53-/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN double knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.
This product corresponds to ATCC Cat. No. CCL-221.
Application
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1.3-1.10
The base medium for this cell line is RPMI, Catalog No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Catalog No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Catalog No. S8636, to a final concentration of 1 mM.
The cell freezing medium is DMSO 1X (Catalog Number C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1.3-1.10
The base medium for this cell line is RPMI, Catalog No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Catalog No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Catalog No. S8636, to a final concentration of 1 mM.
The cell freezing medium is DMSO 1X (Catalog Number C6164).
Features and Benefits
Zinc finger nuclease (ZFN) double knock out on chromosome 17p13.1
The DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
The DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, and PCR assay for cell line species confirmation.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
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