Skip to Content
Merck

Key Documents

View All Documentation

D9785

DMEM/F-12

With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure

Synonym(s):

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.

Preparation Note

Formulated to contain 14.8 grams of powder per liter of medium.
Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

Still not finding the right product?

Explore all of our products under DMEM/Nutrient Mixture F-12 Ham

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

D9785-ALC2: + D9785-50K: + D9785-1L: + D9785-50KG: + D9785-PROC: + D9785-BIBC: + D9785-10L: + D9785-25K: + D9785-10X1L: + D9785-ALC: + D9785-2X5L: + D9785-BULK: + D9785LF1-BIBC: + D9785-VAR: + D9785-25KG: + D9785-50L:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library