DUO82040
Duolink® In Situ Mounting Medium with DAPI
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About This Item
product line
Duolink®
technique(s)
proximity ligation assay: suitable
fluorescence
λex 360 nm; λem 460 nm (Zeiss Filter set 49)
suitability
suitable for fluorescence
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
Use the Duolink® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink®PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Duolink® In Situ Mounting Medium with DAPI is ideal for nuclear staining and preserving signals generated with the Duolink® In Situ Detection Reagents for fluorescence microscopy. See datasheet for more details.
Note: Counterstaining with Cy®2 is not recommended.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Substances Subject to be Notified Names
ishl_notified
DUO82040-BULK: + DUO82040-5ML:
jan
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Articles
細胞および組織中のタンパク質の免疫蛍光検出プロトコル
実験のTips & Tricks、FAQ、基本的なトラブルシューティングなどのサポート情報。
近接ライゲーションアッセイ技術の仕組みと、EGFによるEGFR-HER2二量体化のin situ検出を確認できるタンパク質間相互作用コントロールキットの仕組みについてご紹介します
Duolink™アッセイプロトコルの準備・セットアップ・実施にあたり考慮すべき事項
Protocols
蛍光顕微鏡とMulticolor試薬を使用して、一つのサンプルでタンパク質、タンパク質修飾、もしくはタンパク質間相互作用を最大4つ同時に検出、視覚化そして定量します。
このページでは、蛍光顕微鏡観察のためのDuolink™ In Situの簡易プロトコルを紹介しています。
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