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Merck

E2636

ExtrAvidin® –Alkaline Phosphatase

buffered aqueous solution

Synonym(s):

Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00163600
Form:
buffered aqueous solution

Key Documents

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conjugate

alkaline phosphatase conjugate

form

buffered aqueous solution

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

shipped in

ambient

storage temp.

2-8°C

Quality Level

200

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Application

ExtrAvidin® -Alkaline Phosphatase has been used in:
  • Dot blot
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Indirect ELISA
  • Enzyme linked immunosorbent assay (ELISA)
  • Enzyme-linked immunosorbent spot (ELISPOT) analysis
  • Competitive ELISA

Biochem/physiol Actions

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

E2636-.2ML: + E2636-5X.5ML: + E2636-.5ML: + E2636-BULK: + E2636-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000282616
0000369712
0000529761
0000529761
0000534834

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Alkaline Phosphatase (2103)
C E Rutten et al.
Leukemia, 22(7), 1387-1394 (2008-04-18)
Mismatching for human leukocyte antigen (HLA)-DPB1 in unrelated donor hematopoietic stem cell transplantation (URD-SCT) has been associated with a decreased risk of disease relapse, indicating that HLA-DP may represent a target for graft-versus-leukemia (GVL) reactivity in HLA class II-expressing hematological
Robin B Gore et al.
The Journal of allergy and clinical immunology, 117(3), 649-655 (2006-03-09)
The relationship among inhaled allergen exposure, sensitization, and asthma severity is unknown. To investigate the relationship among personal allergen exposure, reservoir dust allergen concentrations, and physiological measures of asthma severity; to examine the numbers of particles inspired that react with
Jenny Meegan et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 22(6), 856-862 (2010-11-23)
A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal (Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence
W E Sweeney et al.
Kidney international, 56(2), 406-413 (1999-08-05)
Recessively transmitted polycystic kidney disease (PKD) in many murine models is characterized by the initial formation of proximal tubular cysts (stage 1), followed by growth and enlargement of renal collecting tubule (CT) cysts (stage 2). Previous studies have reported that
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Articles

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Protocols

免疫組織染色のプロトコル

パラフィン包埋組織切片中の特定の抗原を染色および可視化する免疫組織染色（IHC）の全プロトコルをご紹介します。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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