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Merck

F4135

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Synonym(s):

HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
MDL number:
MFCD00132239

Key Documents

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Product Name

Fetal Bovine Serum, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

biological source

bovine fetus

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Quality Level

500

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Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
  • as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
  • as a component of blocking medium for treating brainstem slices
  • to improve attachment of dermal explants

General description

Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.

Preparation Note

Prepared by heating at 56°C for 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
19G462
25D106
23L076
24E201
24E012

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Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
Suleymanouglu N, et al.
Journal of Molecular Structure, 1144, 80-86 (2017)
Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis
Akkoc A, et al.
Journal of Applied Animal Research, 46, 445-450 (2018)
Sanjay Ghosh et al.
Nucleic acids research, 44(9), e84-e84 (2016-02-07)
Long non-coding RNAs (lncRNAs) have emerged as regulators of gene expression across metazoa. Interestingly, some lncRNAs function independently of their transcripts - the transcription of the lncRNA locus itself affects target genes. However, current methods of loss-of-function analysis are insufficient
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
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