F4375
Ficoll® PM 400
Type 400
Synonym(s):
Polysucrose 400
Quality Level
biological source
non-animal source
type
Type 400
form
powder, spray-dried
optical activity
[α]20/D 56.5°, c = 1% (w/v) in water
technique(s)
tissue culture: suitable
color
white to off-white
solubility
H2O: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow
suitability
suitable for component for culture media
storage temp.
room temp
General description
Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is very hydrophilic and extremely water-soluble. The most common application for Ficoll 400 is as a density gradient medium for the separation and isolation of eukaryotic cells, organelles and bacterial cells. Density ranges up to 1.2 g/ml can be attained. It has also been utilized in a variety of other applications.
Application
Ficoll® PM 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque a density mixture used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. It may be used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations. Ficoll PM-400 may be used in cell freeze-drying research.
Used for cell separation and organelle isolation.
Features and Benefits
Because of the high molecular weight and low content of dialyzable material, Ficoll has a much lower permeability towards cell membranes than sucrose. Therefore, cells can be expected to collect at a lower density in Ficoll gradients than in sucrose gradients. Because of its low membrane permeability and low osmotic pressure, separations in Ficoll normally result in better preservation of cell function and morphology.
Preparation Note
Concentrations of 50% (w/v) can be attained in water. Ficoll should be added slowly with constant stirring.
Sigma tests the solubility of Ficoll 400 at 1 g in 10 ml of deionized water yielding a clear to slightly hazy, colorless to faint yellow solution. Ficoll is stable in alkaline and neutral solutions. At pH values below 3, it is rapidly hydrolyzed, particularly at elevated temperatures. Ficoll can be sterilized by autoclaving at a neutral pH, at 110oC for 30 minutes. Strong oxidizing and reducing agents are to be avoided.
Sigma tests the solubility of Ficoll 400 at 1 g in 10 ml of deionized water yielding a clear to slightly hazy, colorless to faint yellow solution. Ficoll is stable in alkaline and neutral solutions. At pH values below 3, it is rapidly hydrolyzed, particularly at elevated temperatures. Ficoll can be sterilized by autoclaving at a neutral pH, at 110oC for 30 minutes. Strong oxidizing and reducing agents are to be avoided.
Other Notes
A nonionic synthetic polymer of sucrose.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
F4375-100G:4548173196497 + F4375-50G: + F4375-EW: + F4375-500G:4548173196527 + F4375-VAR: + F4375-25G:4548173196510 + F4375-250G: + F4375-10G:4548173196503 + F4375-BULK:
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Imen Jaballi et al.
International journal of biological macromolecules, 123, 1267-1277 (2018-12-07)
The present study aims to investigate the physicochemical and the structural features of polysaccharide isolated from the red marine macro alga Chondrus canaliculatus (C.C.P) using FT-IR, gel filtration high-pressure chromatograph, HPLC-FID and solid state 13C NMR analysis. C.C.P was even
Samuel G Rodriques et al.
Science (New York, N.Y.), 363(6434), 1463-1467 (2019-03-30)
Spatial positions of cells in tissues strongly influence function, yet a high-throughput, genome-wide readout of gene expression with cellular resolution is lacking. We developed Slide-seq, a method for transferring RNA from tissue sections onto a surface covered in DNA-barcoded beads
Raquel Raizel et al.
The British journal of nutrition, 116(3), 470-479 (2016-05-25)
We evaluated the effects of chronic oral supplementation with l-glutamine and l-alanine in their free form or as the dipeptide l-alanyl-l-glutamine (DIP) on muscle damage, inflammation and cytoprotection, in rats submitted to progressive resistance exercise (RE). Wistar rats (n 8/group)
Chinmayee Sethy et al.
Journal of cancer research and clinical oncology, 146(1), 245-259 (2019-10-17)
In the present study, we have systematically examined the clinical significance of Nectin-4 (encoded by the PVRL-4 gene), a marker for breast cancer stem cells (CSCs), in cancer metastasis and angiogenesis using a variety of human specimens, including invasive duct
Fahad M Almajid
Indian journal of pathology & microbiology, 54(4), 775-781 (2012-01-12)
Seronegative brucellosis is occasionally encountered in clinical practice especially in localized disease where diagnosis is reached mainly through positive blood culture. Cellular immune responses are pivotal for protection against intracellular bacteria such as Brucella. This study was performed to evaluate
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