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F6162

Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal

clone FB-8, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse, Anti-FGF2

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD00212948
Conjugate:
unconjugated
Clone:
FB-8, monoclonal
Application:
DB, ELISA (i), Neutral, RIA, WB
Citations:
18
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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FB-8, monoclonal

form

buffered aqueous solution

mol wt

antigen 18 kDa

species reactivity

bovine, human

technique(s)

dot blot: suitable, indirect ELISA: suitable, neutralization: 5 μg/mL using 1 unit recombinant human FGF2 in mouse 3T3 cells, radioimmunoassay: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P09038

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FGF2(2247)

General description

Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the FB-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Fibroblast growth factors (FGFs) are members of a family of polypeptides. The names FGF acidic and basic are used for FGF-1 and FGF-2, respectively. Acidic and basic fibroblast growth factor (aFGF and bFGF, respectively) are members of a small family of heparin binding growth factors (HBGF). bFGF usually has an apparent mass of about 18 kDa.

Immunogen

recombinant human FGF2.

Application

Anti-Fibroblast Growth Factor-Basic antibody has been used in in immunolabelling and western blotting. It may also be used in:
  • in immunoblotting
  • in enzyme linked immunosorbent assay (ELISA)
  • in dot blot
  • in radio immune assay (RIA)
  • in neutralization bioassays

Used in protein pin array assay to identify the interactive sequences in human αB crystalline protein. The antibody can be used for neutralization of the biological activity of FGF2 in cell culture.

Biochem/physiol Actions

Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activating pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis.
The antibody reacts specifically with both native and recombinant human FGF2. It does not cross-react with human FGF1.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F6162-VAR: + F6162-.2ML: + F6162-BULK:

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