KCQS03
KiCqStart® SYBR® Green qPCR ReadyMix™
iQ™, with fluorescein for Bio-Rad systems
Select a Size
About This Item
form
liquid
usage
sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with Bio-Rad MyiQ, for use with Bio-Rad iCycler iQ, for use with Bio-Rad iQ 5
detection method
SYBR® Green
shipped in
dry ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
- Gene expression
- DNA quantification
- CHiP
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Other Notes
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
KCQS03-SAMPLE: + KCQS03-250RXN: + KCQS03-1250RXN: + KCQS03-5000RXN:
jan
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
Protocols
qPCRにおけるSYBR Green I色素は、ターゲット量を測定し、これは、標準プロトコルで特定のニーズに適合させることができます。
遺伝子発現データの解析には、安定的なリファレンスまたはローディングコントロールが必要です。通常は、1つ以上のリファレンス遺伝子をリファレンスとして使用します
Related Content
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service