M5524
Murashige and Skoog Basal Salt Mixture (MS)
powder, suitable for plant cell culture
Synonym(s):
MS Basal Salts, MS0 Basal Salts, MSO Basal Salts
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About This Item
NACRES:
NA.72
UNSPSC Code:
12352207
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
shipped in
ambient
storage temp.
2-8°C
Quality Level
General description
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).
Application
Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells. It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.
Preparation Note
Formulated to contain 4.3 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
Other Notes
Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.
Store dry medium in a desiccator at 0-5 °C.
Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.
Media Preparation
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.
Store dry medium in a desiccator at 0-5 °C.
Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.
Media Preparation
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
M5524-50L-PW: + M5524-1L: + M5524-25K: + M5524-50K: + M5524-PROC: + M5524-BULK: + M5524-1L-PW: + M5524-10L-PW: + M5524-BIBC: + M5524-50L: + M5524-VAR: + M5524-50KG: + M5524-1L-LBL: + M5524-50L-LBL: + M5524-10L-LBL: + M5524-25KG: + M5524-10L: + M5524-1L-KC:
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Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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