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P2192

10X PCR Buffer

Name: 10<SC>X</SC> PCR Buffer
Brand: SIGMA

Optimized for routine PCR with MgCl₂ included

Synonym(s):

PCR Buffer Solution, PCR Reaction Buffer

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About This Item

NACRES:

NA.52

UNSPSC Code:

41106306

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Properties

Quality Segment

300

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable, whole genome amplification: suitable

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Description

Application

10X PCR Buffer has been used as a component of the PCR mixture:

for the amplification of viral DNA
to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Features and Benefits

Standalone buffer
Compatible with JumpStart^™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq^® Genomic DNA Polymerase (D8312)

Preparation Note

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl₂; 0.01% gelatin

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P2192-1VL-PW: + P2192-BULK: + P2192-1VL: + P2192-5VL: + P2165-2MG-PW: + P2192-5ML: + P2192-25ML: + P2192-EW: + P2192-5VL-PW: + P2192PROC: + P2192-1VL-KC: + P2192-PH: + P2192-25ML-KC: + P2192-VAR: + P2192-25ML-PW: + P2192-5ML-PW:

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