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Merck

P5493

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
MDL number:
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Product Name

Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

grade

BioPerformance Certified
Molecular Biology

sterility

0.2 μm filtered

form

solution

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

impurities

Bioburden, tested
endotoxin, tested
≤5 ppm heavy metals (as Pb)

color

colorless

pH

7.2-7.6 (25 °C)

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for
suitable for flow cytometry
suitable for microscopy

application(s)

advanced drug delivery

foreign activity

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

Quality Level

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Application

Phosphate buffered saline has been used:
  • as a cell wash solution in human dental pulp stem cells (DPSCs)
  • for perfusion studies in dissected brain samples
  • as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling
  • as wash solution for ovary and sperm during in vitro culture and fertilization

Other Notes

For additional information on our range of Biochemicals, please complete this form.
When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P5493PROC: + P5493-1L-PW: + P5493-4L-PW: + P5493-VAR: + P5493-4L: + P5493-BULK: + P5493-PH: + P5493-1L:

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