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P5936

Pectolyase from Aspergillus japonicus

suitable for plant cell culture, lyophilized powder

greener alternative

Greener Alternative

Synonym(s):

PEL1

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About This Item

UNSPSC Code:
10171502
NACRES:
NA.72
EC Number:
3.2.1.15 (BRENDA, IUBMB)
MDL number:
MFCD00131813
Specific activity:
≥0.3 unit/mg solid
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form

lyophilized powder

Quality Segment

200

specific activity

≥0.3 unit/mg solid

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

cell culture | plant: suitable

application(s)

agriculture

greener alternative category

Enabling,

storage temp.

2-8°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.
Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P5936-1G: + P5936-250MG: + P5936-EW: + P5936-VAR: + P5936-100MG: + P5936-25MG: + P5936-BULK:

jan

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