Skip to Content
Merck

P8169

Phytagel™

BioReagent, suitable for plant cell culture, powder

Synonym(s):

Gellan Gum, Agar substitute gelling agent

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
71010-52-1
UNSPSC Code:
10171502
EC Number:
275-117-5
NACRES:
NA.72

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

product line

BioReagent

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.

Application

Phytagel™ has been used:
  • in the solidification of culture medium for root assays in Arabidopsis thaliana
  • as a component of Solid Paul′s medium for Agrobacterium culture
  • as a component of root induction medium in Ilex dumosa tissue culture

Biochem/physiol Actions

Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

Preparation Note

To prevent clumping, add slowly to rapidly stirring medium prior to heating.

Other Notes

An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Legal Information

Phytagel is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P8169-100G: + P8169-VAR: + P8169-1KG: + P8169-500G: + P8169-5KG: + P8169-250G: + P8169-BULK:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000527727
0000527724
0000529060
0000527724
0000529060

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Abhishek Bhaskaran et al.
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 19(5), 874-880 (2016-05-22)
Longer procedural time is associated with complications in radiofrequency atrial fibrillation ablation. We sought to reduce ablation time and thereby potentially reduce complications. The aim was to compare the dimensions and complications of 40 W/30 s setting to that of
Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells
Buschmann H, et al.
The New phytologist, 190(1), 258-267 (2011)
Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Luna C, et al.
Biocell, 27(2), 205-212 (2003)
CEP genes regulate root and shoot development in response to environmental cues and are specific to seed plants
Delay C, et al.
Journal of Experimental Botany, 64(17), 5383-5394 (2013)
Rory N Pruitt et al.
The New phytologist, 215(2), 725-736 (2017-05-31)
The biotrophic pathogen Xanthomonas oryzae pv. oryzae (Xoo) produces a sulfated peptide named RaxX, which shares similarity to peptides in the PSY (plant peptide containing sulfated tyrosine) family. We hypothesize that RaxX mimics the growth-stimulating activity of PSY peptides. Root length
View All Related Papers

Protocols

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service