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R3025

Anti-Rho-GDI antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL

Synonym(s):

Anti-GDIA1, Anti-HEL-S-47e, Anti-NPHS8, Anti-RHOGDI, Anti-RHOGDI-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01634148
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
9
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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

mouse, bovine, human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 4 μg using 500 μg of Jurkat RIPA lysate, western blot: 0.5-1.0 μg/mL using RIPA lysates from human HL-60, HeLa, Hs294T, Jurkat and murine 3T3/NIH cells and mouse 3T3/A31

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ARHGDIA(396)
mouse ... Arhgdia(192662)

General description

Rho guanine nucleotide dissociation inhibitor I (RhoGDI), also known as Rho GDP dissociation inhibitor α (ARHGDIA) is encoded by the gene mapped to human chromosome 17q25.3-25-3. RhoGDI is the member of Rho/Rac protein family. The encoded protein consists of 204 amino acids and has a molecular mass of 23,421,000 Mr. In mammals, three RhoGDI isoforms exists: RhoGDIα, RhoGDIβ and RhoGDIγ. RhoGDIα is widely expressed. RhoGDIβ is mainly expressed in hematopoietic cells and RhoGDIγ is present in the brain, lung, kidney and testis.
Rho proteins are basically described as guanine nucleotide-binding proteins (GNBPs). RhoGDI (Rho guanine nucleotide dissociation inhibitor)/ARHGDIA (Rho guanine nucleotide dissociation inhibitor) has three subtypes namely- RhoGDIα (Rho GDP dissociation inhibitor α), RhoGDIβ (Rho GDP dissociation inhibitor β) and RhoGDIγ (Rho GDP dissociation inhibitor γ). RhoGDIα is expressed everywhere. It is located on human chromosome 17q25.3. Its protein has 204 amino acids and belongs to the Rho/Rac family.

Immunogen

GST fusion protein corresponding to full-length human Rho-GDI.

Application

Anti-Rho-GDI antibody produced in rabbit has been used in immunoblotting.
Anti-Rho-GDI has been used in western blotting.

Biochem/physiol Actions

Rho proteins control the actin and microtubule cytoskeleton. RhoGDIα is necessary for the spatial and temporal modulation of RhoGNBPs (guanine nucleotide-binding proteins). In HCC (hepatocellular carcinoma) patients, absence of ARHGDIA (Rho guanine nucleotide dissociation inhibitor) affects the prognosis and induces invasion and metastasis of HCC cells.
The antibody recognizes Rho-GDI and cross-reacts with D4-GDI.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.

Preparation Note

Purified using protein A

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

R3025-200UG: + R3025-200UG-PW: + R3025-.1MG: + R3025-VAR: + R3025-BULK:

jan

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