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R4875

Ribonuclease A from bovine pancreas

Name: Ribonuclease A from bovine pancreas
Brand: SIGMA

Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

Synonym(s):

Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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About This Item

CAS Number:

9001-99-4

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-646-6

MDL number:

MFCD00132181

EC Number:

3.1.27.5 (BRENDA, IUBMB)

eCl@ss:

32160703

Specific activity:

≥50 Kunitz units/mg protein

Assay:

≥60% (SDS-PAGE)

Biological source:

bovine pancreas

Concentration:

≥60% (RNase A, SDS-PAGE)

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Properties

biological source

bovine pancreas

Quality Segment

300

type

Type I-A

assay

≥60% (SDS-PAGE)

form

powder

specific activity

≥50 Kunitz units/mg protein

mol wt

~13,700

concentration

≥60% (RNase A, SDS-PAGE)

technique(s)

DNA purification: suitable, cell based assay: suitable

impurities

salt, essentially free

input

general use sample(s)

optimum pH

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

foreign activity

protease, essentially free

storage temp.

−20°C

SMILES string

[nH]1cncc1CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Substrate Specificity

an [RNA] containing cytidine + H₂O = an [RNA]-3′-cytidine-3′-phosphate + a 5′-hydroxy-ribonucleotide-3′-[RNA] || an [RNA] containing uridine + H₂O = an [RNA]-3′-uridine-3′-phosphate + a 5′-hydroxy-ribonucleotide-3′-[RNA]

Application

RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
RNase A is also used in RNA sequence analysis and protection assays.
RNase A has been used as a tool for computer-aided drug design.
RNase A supports the analysis of RNA sequences.
RNase A hydrolyze RNA contained in protein samples.
Purification of DNA is supported by RNase A.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His¹² and His¹¹⁹ and activated by potassium and sodium salts.

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Preparation Note

Salt fractionated and chromatographically purified.

Analysis Note

Protein determined by E.

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)