S1452
MISSION® siRNA Transfection Reagent
Synonym(s):
MISSION® siRNA Transfection Reagent
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About This Item
NACRES:
NA.51
UNSPSC Code:
41106502
General description
The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Other Notes
Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
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Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
120.2 °F
flash_point_c
49 °C
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Group 4: Flammable liquids + Type 2 petroleums + Hazardous rank III + Water insoluble liquid
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Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
S1452-1ML:4.548173925479E12 + S1452-100UL:4.548173925462E12
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