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S7901

Stem Cell Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, cell culture | mammalian: suitable

Synonym(s):

SCF, c-Kit ligand

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00165757
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
powder
Mol wt:
monomer 18.6 kDa ((165 aa))
Impurities:
≤1 EU/μgtested (LAL test)
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Product Name

Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

powder

potency

≤15 ng/mL ED50

quality

endotoxin tested

mol wt

monomer 18.6 kDa ((165 aa))

packaging

pkg of 10 μg, pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μgtested (LAL test)

UniProt accession no.

P21583

storage temp.

−20°C

General description

Recombinant human Stem Cell Factor (SCF), also known as c‑Kit ligand, is expressed in E. coli and supplied as a lyophilized powder for use in mammalian cell culture research in vitro.

Application

Stem Cell Factor human has been used in
  • In vitro differentiation of equine embryonic stem cells
  • Chemotaxis detection system
  • Ex vivo expansion of umbilical cord blood CD34+ cells
  • Cytokine therapy

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), has broad effect on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. In the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Physical form

Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

S7901-10UG: + S7901-50UG-PW: + S7901-10UG-PW: + S7901-50UG: + S7901-VAR: + S7901-BULK:

jan

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