T9424
TRI Reagent®
For processing tissues, cells cultured in monolayer or cell pellets
Synonym(s):
TRI Reagent® RNA Isolation Reagent
Product Name
TRI Reagent®, For processing tissues, cells cultured in monolayer or cell pellets
usage
mL sufficient for 107 cells
mL sufficient for 100 mg tissue (or)
storage temp.
2-8°C
Quality Level
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Application
- in the isolation of total RNA from various tissue samples and cells
- for the lysis of monocytes
- to isolate cytoplasmic RNA from SARS-CoV infected Vero cells
- to isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus
- to prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine
The RNA, DNA and protein that are isolated using TRI reagent® can further be used for downstream applications, such as cloning, PCR, RT-PCR, Northern blots, mRNA isolation, in vitro translation, RNase protection assay, restriction enzyme digestion, Southern blots, SDS-PAGE and western blots.
Features and Benefits
Multi-purpose: TRI Reagent® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples.
Efficient: TRI reagent® gives better yields than traditional guanidine thiocyanate/cesium chloride methods. The whole process of RNA extraction starting with fresh tissue or cells can be completed in less than one hour.
- Easily scalable RNA isolation
- Works with many sources: human, plant, yeast, bacterial, or viral
- Better yields than traditional guanidine thiocyanate/cesium chloride methods
General description
Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.
Legal Information
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
supp_hazards
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
174.2 °F - closed cup
flash_point_c
79 °C - closed cup
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Deleterious substance
pdsc
Class I Designated Chemical Substances
prtr
Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water insoluble liquid
fsl
Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
T9424-6X100ML:4548173214603 + T9424-100ML:4548173214573 + T9424-BULK: + T9424-25ML:4548173214597 + T9424-200ML:4548173214580 + T9424-VAR:
jan
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Articles
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Protocols
TRI Reagent®では、サンプル調製ガイドラインとトラブルシューティングを用いてDNA、RNA、タンパク質の同時分離が実現します。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Related Content
細胞溶解およびタンパク質抽出法では、界面活性剤による可溶化から機械的破壊まで、さまざまな技術を概説し、研究ニーズをサポートします。
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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